Home biospectra, inc hepes, free acid biological buffer. The free acid can be added to water, then titrated with sodium hydroxide or potassium hydroxide to the ph desired. Carl roth, the expert consultation for labware, life science and chemicals for more than 140 years. Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. N2hydroxyethylpiperazinen2ethanesulfonic acid, 42hydroxyethyl1piperazineethanesulfonic acid. Hepes, free acid, molecular biology grade cas 7365459 calbiochem. Make sure this fits by entering your model number product is sold for research or further manufacturing use only, not for food or drug use. Promega manufacturing and delivery systems continue to be fully operational during the covid19 outbreak. Please note that we cannot offer bulk quantities for some. We are happy to announce that we are fully compliant with ghs requirements domestically as well as internationally. Hepes, free acid, molecular biology grade cas 7365459 calbiochem cas 7365459 a zwitterionice nsubstituted aminosulfonic acid buffer. With more than 30,000 articles available and a roundtheclock delivery service within germany, we are the partner that research, scientific and technical laboratories can rely on.
Other companies are charging thousands of dollars to set up accounts and give you access to their msds online database. Adsorption, wash and regeneration buffer elution buffer company. Hold eyelids apart and flush eyes with plenty of water for at least 20 minutes. Find msds or sds, a coa, data sheets and more information. Hepes is a buffer this means that it will keep your solutions at or close to the ph you require. Have eyes examined and tested by medical personnel. Find where to buy products from australian suppliers, including. It is commonly added at concentrations between 10 mm and 25 mm. Items available from stock in japan will be delivered in 12 weeks. Download msds pdf search msds currently available for download bioworld offers hepes free acid for your research at low price. Hepes, free acid biological buffer biospectra, inc cphi. View or download the hepes, free acid, ultrol grade cas 7365459 calbiochem msds material safety data sheet or sds for 3938 from milliporesigma. Hepes free acid a buffer is most effective when the ph is equal to the pka of that buffer, and most efficient when in the range of one ph unit above and below that value.
Hepes is used in many media because it has more buffering capacity in the physiologic ph range of 7. Our teams are in regular contact with suppliers and distributors worldwide and are taking all steps necessary to address both demands for diagnostic tools and reliable delivery of all products as quickly as possible. Hepes, free acid, ultrol grade, 1 m solution cas 7365459. Thus its recommended to use dnasernaseprotease free hepes biosciencegrade as soon as molecular biological applications like pcr, extraction, isolation, hybridization etc.
Your new online msds binder is a place for you to store the material safety data sheets you need to deploy. Galab technologies gmbh am schleusengraben 7 21029 hamburg germany. View product specific information, msds, references and buying faq. View or download the hepes, free acid, molecular biology grade cas 7365459 calbiochem msds material safety data sheet or sds for 3940 from merck. Hepes, free acid, molecular biology grade cas 7365459. Hepes is commonly used to uphold a neutral ph of basal medium within cell cultures. Alternatively, equimolar concentrations of hepes and of sodium hepes sc216092 can be mixed in approximately equal volumes, backtitrating with either solution to the appropriate ph. Hepes, free acid, molecular biology grade cas 7365459 calbiochem a zwitterionice nsubstituted aminosulfonic acid buffer. Safety data sheet for hepes, free acid, ultrol grade, 1 m solution cas 7365459 calbiochem 375368.
Material safety data sheet or sds for hepes, free acid, ultrol grade cas 7365459 calbiochem 3938 from milliporesigma for download or viewing in the browser. Learn more about hepes 242hydroxyethyl1piperazinylethanesulphonic acid. Hepes free acid molecular biology 42hydroxyethylpiperazine1ethanesulfonic acid, n2hydroxyethylpiperazinen2ethanesulfonic acid c8h18n2o4s. Identification of the substancemixture and of the companyundertaking product name. Hepes 42hydroxyethylpiperazin1ylethanesulphonic acid. Standard and custom formulations for lab and process scales. Outsource your microbiology media and biological buffers and solutions preparation. View or download the hepes, free acid, ultrol grade, 1 m solution cas 7365459 calbiochem msds material safety data sheet or sds for 375368 from merck. When higher ph values are needed, or to keep the ph steady, hepes. Hepes, free acid, ultrol grade, 1 m solution cas 7365. The addition of 10 25 mm hepes provides extra buffering capacity when cell culture requires extended periods of manipulation outside of a co2 inc. Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids.
Hepes, sodium salt is a zwitterionic goods buffer used to prevent ph changes caused by co2 variability and is a common alternative buffer to bicarbonate. Hence, hepes is particularly adapted for cell and tissue cultures. Hepes 42hydroxyethyl1piperazineethanesulfonic acid is a zwitterionic sulfonic acid buffering agent. Gibco hepes n2hydroxyethylpiperazinen2ethane sulfonic acid is a zwitterionic organic chemical buffering agent commonly used in cell culture media. We enable science by offering product choice, services, process excellence and our people make it happen. Hepes, free acid is a buffer for aqueous solutions with a pka 7. Hepes revision date 18jan2018 upper no data available lower no data available sensitivity to mechanical impactno information available sensitivity to static dischargeno information available specific hazards arising from the chemical noncombustible, substance itself does not burn but may decompose upon heating to produce corrosive andor.
Irvine scientific supplies material safety data sheets msds for products you have purchased. First aid measures description of first aid measures. The regulations promulgated by osha for hazard communication, 29 cfr 1910. Hepes, free acid, ultrol grade cas 7365459 calbiochem. Hepes is a zwitterionic nsubstituted acidic buffering substance used for culturing cells in vitro. Hepes is widely used in cell culture, largely because it is better at maintaining physiological ph despite changes in carbon dioxide concentration produced by aerobic respiration when compared to. Hepes is also useful in transfections of mammalian cell lines. It is also a component of tris hepes buffer for polyacrylamide electrophoresis of proteins. It seems to me that you should prepare a solution for each ph you require. Learn more about hepes 42hydroxyethylpiperazin1ylethanesulphonic acid. Search for products or services, then visit the suppliers website for prices, sds or more information. Hepes is a zwitterionic buffer used to maintain ph of media used in cell cultures.
Download msds pdf search msds currently available for download bioworld offers hepes buffer 1m, ph 8. Hepes solution is part of highperformance line of nutritional and growth supplements. Hepes free acid, 42hydroxyethylpiperazin1ylethanesulfonic acid suitable for use in biopharmaceutical manufacturing applications. Content % classification signal word hphrases pphrases cacl 2 2h 2o 10035048 2331408 1,3 warning h319 p305 p351 p338 mncl 2 4h 2o 446349 2318696 1,6 warning h302 h4 4. It is also a component of trishepes buffer for polyacrylamide electrophoresis of proteins. Hepes, free acid biological buffer biospectra, inc.
Our laboratory division offers a diverse range of over 6,000 quality reagents and standards, marketed to research, institutional, education and industrial laboratories. Hepes revision date 18jan2018 upper no data available lower no data available sensitivity to mechanical impactno information available sensitivity to static dischargeno information available specific hazards arising from the chemical noncombustible, substance itself does not burn but may decompose upon heating to produce corrosive andor toxic fumes. Hepes has been described as one of the best allpurpose buffers available for biological research. Hepes, free acid, molecular biology grade cas 7365459 calbiochem, n2hydroxyethylpiperazinen.